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Protein Markers and Ladders

WM1000 - YesBlot Western Marker I, 250ul

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Starting at: $165.00

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The WM1000 YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. YesBlot™ Western Marker I performs dual functions: 

  • Contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size
  • Ten IgG-binding proteins can be immuno-detected on film or by CCD imaging

YesBlot™ Western Marker I can be used with chemiluminescent, fluorescent, chromogenic and other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

Specifications:
  • Band Number (Western Bands) - 10 
  • Band Number (Pre-stained Bands) - 4 
  • MW Range (Western Bands) - 15 – 200 kDa
  • MW Range (Pre-stained Bands) - 10 – 70 kDa
  • Pre-stained Band Color - Pink/Green/Blue

Features

  • Includes ten IgG-binding proteins
  • Ready-to-use, supplied in gel loading buffer
  • Can be used with chemiluminescent, fluorescent, chromogenic and other detection systems
  • Two reference bands have enhanced intensity (at 30 kDa and 80 kDa)
  • Contains recombinant IgG binding proteins, Glycerol, SDS, and tracking dyes in a Tris-HCl buffer
  • Performs dual functions
  • Ten IgGbinding proteins can be immuno-detected on film or by CCD imaging

Applications

  • Monitoring protein separation during SDSPAGE
  • Verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) 
  • Approximating protein size

Contents

  • WM1000 YesBlot™ Western Marker I, 250 µl