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Whole Genome Amplification Kits

108725 - WGA Kit, 25 reactions

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Starting at: $445.00

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The One Step Whole Genome Amplification Kit is an improved version of the DOP (degenerated oligo PCR) with optimized primer sequences and Bioron’s unique SD Polymerase. This enzyme combines the strand displacement capability with heat stability up to 92°C. Due to these features, it is possible to use this enzyme for both amplification steps without additional pipetting or handling. All components necessary for amplification of human genomic DNA from single cells or low copy templates are included. The WGA Kit is designed for reproducible amplification of low copy number genomic DNA to yield 2-10 µg of product in about 2 hours.

Features

  • Amplification finished in less than 2 hours
  • Different genomic amplification coverage than other enzymes
  • Long fragments up to 3 Kb
  • One Enzyme for the whole genome amplification
  • Ready for PCR and NGS
  • Suitable for single cell DNA Analysis

Applications

  • Single cell DNA analysis
  • Next Generation Sequencing (NGS)
  • SNP Genotyping
  • qPCR- and PCR-based mutation detection
  • STR and RFLP analysis
  • Array technologies (comparative genomic hybridization)

Contents

  • 2.5x Master Mix without Enzyme, 250 µl
  • 10 U/µl SD Polymerase, 25 µl
  • PCR grade H2O, 1800 µl
  • Human genomic DNA as positive control, 70 µl

Other Sizes Available: 50 reactions

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