Home New Customer? Create Account
Member Login:

Magnetofection Magnetic Transfection

FV10100 - ViroMag/FluoMag-V Red Fluorescent Magnetofection Reagent, 100μl

PRICE

Starting at: $163.00

Please Choose:

Shipping Option



Enter Quantity:
FluoMag Transfection Reagents are fluorescently-labeled magnetic nanoparticles for Magnetofection applications. They are as efficient as their non-labeled counterparts and allow the visualization of the nanoparticles in vitro during an experiment. 

FluoMag-V corresponds to ViroMag, a viral transduction reagent. This reagent must be used with a magnetic plate.


See also:
  • FluoMag-C, corresponds to CombiMag (transfection reagent enhancer)
  • FluoMag-S, corresponds to SilenceMag (siRNA delivery)
  • FluoMag-P, corresponds to PolyMag (DNA transfection reagent)



Figure 1: NIH-3T3 transfection with FluoMag-P and pEGFP plasmid DNA. NIH-3T3 cells (5x104 cells/well), growing on coverslips, were transfected in 24-well plates with 1 µg of pEGFP plasmid and 1µL of  FluoMag-P per well as described in the Magnetofection instruction manual. At several times post-transfection, cells were fixed, stained with DAPI to detect nucleus (blue) and observed under a fluorescent microscope equipped with a CCD camera.


Figure 2: Transfection efficiency of FluoMag-S versus SilenceMag. GFP stably transfected MDCK and HeLa cells were plated the day before transfection in a 24-well plate. Cells were then treated with SilenceMag or FluoMag-S and siRNA (targeting GFP or targeting LacZ as control) as described in the SilenceMag instruction manual. Complexes were prepared with 1 μL of SilenceMag or FluoMag-S and 10nM (67.5ng) of siRNA. Cells were then transfected in 500 μL transfection volume. GFP expression level was monitored 72h post-transfection by detection of fluorescence intensity with a fluorometer. 

Features

  • Easy cell labeling and tracking
  • Non-toxic
  • Compatible with serum

Applications

  • Double labeling and co-localization studies using GFP or FITC labeled nucleic acids 
  • Transfection mechanisms 
  • Fluorescent resonance energy transfer (FRET) assay
  • Analysis of association of vectors with magnetic nanoparticles

Contents

  • FluoMag-V, 100 µl