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Magnetofection Magnetic Transfection

RL40000 - ViroMag R/L Viral Gene Delivery Reagent (Retrovirus/Lentivirus)

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ViroMag R/L Transduction Reagent is a magnetic nanoparticles formulation optimized for Retroviruses and Lentiviruses. Based on Magnetofection technology, this reagent allows concentration of the complete applied dose of Retro/Lentiviral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses. Due to its specific properties, ViroMag R/L is ideal for infecting non-permissive cells. It demonstrates an exceptionally high efficiency in promoting, controlling and assisting viral transductions. No molecular biology processes or biochemical modifications are required. This reagent must be used with a magnetic plate.


Figure 1: Protocol. In the process of Magnetofection, nucleic acids, transfection reagents or viruses are associated with specific magnetic nanoparticles. The resulting molecular complexes are then concentrated and transported into cells supported by an appropriate magnetic field. 

Figure 2: ViroMag R/L is highly efficient for lentiviral infection. NIH-3T3 were infected with a lentivirus coding for GFP alone or with Polybrene, ViroMag and ViroMag R/L. Percentage of infected cells was determined 48 and 72H after infection by FACS analysis.

Figure 3: ViroMag R/L is highly efficient in a dose dependent manner. NIH-3T3 were infected with a lentivirus coding for GFP in presence of several doses of ViroMag R/L. Percentage of infected cells was determined 48 and 72H after infection by FACS analysis.

Features

  • Designed for use with retroviruses and lentiviruses
  • Increases transduction efficiency
  • Accelerates the transduction process
  • Efficient for hard-to-infect and non-permissive cells
  • Concentrates viral dose onto the cells
  • Synchronizes cells adsorption/infection without requiring modifications of the viruses

Applications

  • Cell transduction with retroviral and lentiviral vectors, especially VSV-G pseudoviruses

Sizes

  • RL40100 – ViroMag R/L Transfection Reagent, 100 µl (30-500 transductions)
  • RL40200 – ViroMag R/L Transfection Reagent, 200 µl (60-1,000 transductions)
  • RL41000 – ViroMag R/L Transfection Reagent, 1ml (300-5,000 transductions)

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