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Genomic DNA Extraction

17151 - Viral Gene-spin DNA/RNA Extraction Kit, 50 preps

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$221.00

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The Viral Gene-Spin Kit is designed for rapid isolation of viral RNA or DNA from a variety of sample sources including fresh or frozen plasma, serum, other cell-free body fluids and virus-infected cells/tissue. Using advanced silica-gel membrane technology, RNA or DNA can be purified without organic extraction or ethanol precipitation. The buffering conditions are finely adjusted to provide optimum binding of the RNA/DNA to the column. The purified RNA/DNA is free of contaminants and impurities. The protocol for this kit is simple and can be completed in 20 minutes.

Protocol

protocol

Results of viral RNA extraction from various sources

gels

Application to "Novel H1N1"

h1n1

Features

  • Extracts viral DNA/RNA from a variety of clinical samples within 20 minutes
  • Powerful lysis buffer creates high yield and purity
  • Easy extraction without enzyme treatment or ethanol precipitation
  • Contamination is minimized by CAPS
  • Especially useful for clinical diagnosis of people or animals that have been infected by viruses or bacteria

Applications

  • Pathogen detection
  • Microarray
  • cDNA synthesis
  • Reverse Transcriptase PCR (RT-PCR)
  • Quantitative PCR (qPCR, qRT-PCR)

Contents

  • Lysis buffer, 30ml
  • Binding buffer, 40ml
  • Washing buffer A, 30ml
  • Washing buffer B, 10ml
  • Elution buffer, 20ml
  • Spin columns x 50
  • Collection tubes x 50

Literature