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NICE® Expression System for Lactococcus lactis

 
MODEL PRODUCT NAME- PRICE
ELV00100-01 NICE® pNZ8008 Reference plasmid with gusA gene, 10 µg $827.00
ELV00200-01 NICE® pNZ8148 Lactococcus lactis expression vector, NcoI site, 10 µg $827.00
ELV00300-01 NICE® pNZ8149 Lactococcus lactis expression vector, NcoI site, food grade, 10 µg $827.00
ELV00250-01 NICE® pNZ8150 Lactococcus lactis expression vector, ScaI site, 10 µg $827.00
ELV00600-01 NICE® pNZ8120 Lactococcus lactis secretion vector, NaeI site, 10 µg $1,050.00
ELV00650-01 NICE® pNZ8121 Lactococcus lactis secretion vector, EcoRV site, 10 µg $1,050.00
ELV00700-01 NICE® pNZ8122 Lactococcus lactis secretion vector, NruI site, 10 µg $1,050.00
ELV00750-01 NICE® pNZ8123 Lactococcus lactis secretion vector, Nae site, 10 µg $1,050.00
ELV00800-01 NICE® pNZ8124 Lactococcus lactis secretion vector, EcoRV site, 10 µg $1,050.00
ELV00500-01 NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in strain NZ9000, 1ml $504.00
ELS09100 NICE® Lactococcus lactis expression strain NZ9100, 1ml $544.00
ELS03900-01 NICE® Lactococcus lactis expression strain NZ3900, food grade, 1ml $504.00
ELS03910-01 NICE® Lactococcus lactis expression strain NZ3910, food grade, 1ml $544.00
ELS09000-01 NICE® Lactococcus lactis expression strain NZ9000, 1ml $504.00
ELS09130-01 NICE® Lactococcus lactis expression strain NZ9130, food grade, 1ml $657.00
ELS10710-01 MC1061 Chemically Competent E. coli, 100µl x 5 $751.00
ELS10610-01 NICE® E. coli host strain MC1061, 1ml $523.00
ELK01000-02 NICE® Nisin Kit $240.00

NICE® Expression System for Lactococcus lactis

The vector Lactococcus lactis is increasingly used for modern biotechnological applications such as the production of recombinant proteins for food, feed, pharma and biocatalysis applications. The Nisin Controlled gene Expression (NICE®) system has played an important role in this development. It can be used for over expression of homologous and heterologous genes for functional studies and to obtain large quantities of specific gene products. The major advantages of the NICE® system over other expression systems are the expression of membrane proteins, secretion of proteins into the medium, tightly controlled gene expression and simple scale up and downstream processing. Please see below for an overview of the NICE® vectors. If you do not see the product you are looking for, please contact us.

Please note, a license is required for any non-academic customer.  Please contact us for more information.


See below for an overview of the NICE® L. lactis vectors.


Cat #

Vector

Cloning Site

Secretion

Signal Peptide

Food Grade

ELV00100-01

pNZ8008*

n/a

No

No

No

ELV00200-01

pNZ8148

NcoI

No

No

No

ELV00250-01

pNZ8150

ScaI

No

No

No

ELV00300-01

pNZ8149

NcoI

No

No

Yes

ELV00500-01

pNZ9530**

n/a

No

No

No

ELV00600-01

pNZ8120

NaeI

Yes

PrtP

No

ELV00650-01

pNZ8121

EcoRV

Yes

PrtP

No

ELV00700-01

pNZ8122

NruI

Yes

SlpA

No

ELV00750-01

pNZ8123

NaeI

Yes

Usp45

No

ELV00800-01

pNZ8124

EcoRV

Yes

Usp45

No


*Reference plasmid carrying gusA gene
**Carries nisR/nisK for expression in strains without nis regulating elements