Home New Customer? Create Account
Member Login:

Master Mixes

119112 - Universal Master Mix (2x), 200 reactions

PRICE

Starting at: $91.00

Please Choose:

Shipping Option



Enter Quantity:
Universal Master Mix 

Universal Master Mix (2x) is a ready-to-use PCR mixture of HotStart Taq DNA Polymerase, PCR buffer, MgCl2 and dNTPs. 2x PCR Master Mix contains all components for PCR, except sample DNA and primers. The mixture is suited for regular PCR as well as for DNA intercalating fluorescent dye (Sybr Green, Eva Green, etc) and probe based Real Time PCR. However, Real Time PCR assays with the Universal Master Mix may need customer-specific optimization.

Features:
  • High sensitivity & specificity
  • HotStart Taq DNA Polymerase of Bioron included
Applications:
  • Real Time PCR
  • qPCR
  • RT-PCR
  • End point analysis
Performance and purity tests:
  • Tested for the absense of endodeoxyribonucleases and exodeoxyribonucleases
  • The Universal Master Mix is tested in the amplification of a single-copy gene of mouse genomic DNA
  • Endodeoxyribonuclease Assay: No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 25µl of 2x Universal Master Mix with 1µg of pUC19 DNA in 50µl for 4 hours neither at 37C nor at 70C.
  • Universal Master Mix is twofold concentrated 2 x 1.25ml of Master Mix is enough for 200 reactions in 25µl final reaction volumne