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GRiSP Research Solutions - PCR & qPCR, RNA Research, and Sample Purification

GCM19.0500 - Terrific Broth, Auto Induction Medium, 500g

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Terrific Broth (Auto Induction Medium) is a dehydrated powder for the preparation of Terrific Broth without trace elements. This medium is supplemented with glucose and alpha lactose for the auto induction of protein expression under the control of IPTG-inducible promoters in E. coli. The Auto Induction Medium (AIM) provides an efficient means of protein expression that requires far less attention by the user than conventional IPTG induction.

Heterologous protein expression is often carried out in bacterial systems in which the expression is under the control of an IPTG-inducible promoter, such as the Lac promoter. Cells are grown until a desired density and protein expression is reached and subsequently induced by adding IPTG to the medium. With the Auto Induction Medium (AIM), it is no longer necessary to monitor cell density and to add IPTG at the proper stage, as the medium contains an optimized ratio of glucose and alpha lactose as carbon sources. Glucose, serving as a repressor of the Lac operon, prevents uptake of alpha lactose (hence and IPTG) and is metabolized preferentially during growth, promoting high cell density. Once glucose is depleted, lactose enters the cell where it is converted by ßgalactosidase into allolactose. Allolactose in turn serves as the inducer of the IPTG-inducible promoter, resulting in protein expression. This system offers unmatched convenience and simplifies manual or automatic induction and analysis of multiple clones compared to conventional IPTG induction.

Formulation (g/l)
  • Tryptone: 12.00
  • Yeast Extract: 24.00
  • MgSO4: 0.15
  • (NH4)2SO4: 3.30
  • KH2PO4: 6.50
  • Na2HPO4: 7.10
  • Glucose: 0.50
  • Alpha Lactose: 2.00
  • Final pH (25⁰C): 7.0 ± 0.2

Preparation
Add 55.55g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute. Dispense in appropriate containers and sterilize by autoclaving at 121⁰C for 15 to 20 minutes. Store at 2-8⁰C.

Features

  • Supplemented with glucose and alpha lactose for auto induction of protein expression
  • Does not require the user to monitor cell density due to glucose and alpha lactose 
  • Simple and convenient compared to conventional IPTG induction

Contents

  • Terrific Broth (Auto Induction Medium), 500g