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Master Mixes

101600 - Taq Master Mix

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Taq Master Mix


Description:
2x Taq Master Mix is an optimized ready-to-use PCR mixture of Taq DNA Polymerase, PCR buffer, MgCl2, and dNTPs. 2x Taq Master Mix contains all of the components for PCR, except DNA template and primers.

Features:
  • Bioron Taq DNA Polymerase is included
  • High sensitivity
  • Efficient amplification of single copy genes
  • High yield of DNA
  • Without hotstart
  • qPCR
Applications:
  • Real-Time PCR
  • RT-PCR
  • PCR with bacterial DNA
  • REgular PCR up to 5kb

Performance and Purity Tests:
Tested for the absence of endodeoxyribonucleases and exodeoxyribonucleases. Performance is tested on single-copy genes of human and mouse DNA.

Endodeoxyribonuclease Assay:
No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 25l of 2x PCR Master Mix with 1g of pUC19 DNA in 50l for 4 hours neither at 37C nor at 70C

Storage:
Master Mix should be stored at -20C.
Repeated (up to 10 times) freezing - defreezing does not cause reduction in PCR performance.
Storage at 4C for 1 week does not cause reduction in PCR performance.