Home New Customer? Create Account
Member Login:

DNA Polymerases/SNPase

108220 - SNPase DNA Polymerase, Hotstart

PRICE

Starting at: $135.00

Please Choose:

Size



Shipping Option



Enter Quantity:
SNPase HotStart DNA Polymerase is the HotStart version of Klen SNPase. It is designed for cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing as well as for manual DNA sequencing with 35S label. This enzyme has only 5 ‘- 3’ polymerase activity and is also recommended for SNP genotyping by allele-specific PCR (AS-PCR), allele-specific primer extension (AS-PEX) and mini-sequencing procedures. The relative mutation rate during polymerization is 10-15 fold lower for Klen SNPase compared with Taq DNA polymerase.


Features

  • High fidelity of dNTPs and ddNTPs incorporation
  • High specificity leads to low level of background PCR

Applications

  • Cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing or manual DNA sequencing
  • SNP genotyping with AS-PCR, AS-PEX and minisequencing procedures

Sizes


Contents

108210

108250

SNPase HotStart DNA Polymerase

500 units

2500 units

Incomplete Reaction Buffer (10x) 

1.25ml

1.25ml x 2

MgCl2 100 mM

1ml

1ml x 2



Literature