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VS-FLP10010 - pwt-mitoEosFP, with mitochondrial targeting signal, 10µg

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pwt-mitoEosFP (with mitochondrial targeting signal) is an expression vector encoding for the fluorescent green-to-red photoconvertible wildtype EosFP protein (wt-EosFP) already attached to a mitochondrial targeting signal. This fusion protein is functionally expressed in a wide range of pro- and eukaryotic cells at a temperature of 37°C and below. The construct pwt-mitoEosFP (with mitochondrial targeting signal) can be used directly for transfection or microinjection and subsequent analyses by fluorescence microscopy. The mitochondria or parts thereof can be labeled by photoconversion and their movement can be monitored. The photoconversion feature also enables the use of high resolution fluorescence microscopy such as PALM.

Features

  • Expression vector for fluorescent tetrameric wt-EosFP with mitochondrial targeting signal
  • Ready-to-use construct for transfection and subsequent microscopy analyses
  • Green-to-red photoconvertible
  • Permanent, bright and fast
  • Applicable to high resolution fluorescence microscopy such as PALM

Applications

  • Labeling and tracking of mitochondria
  • Selective marking of selected mitochondria by photoconversion

Contents

  • pwt-mitoEosFP, with mitochondrial targeting signal, lyophilized DNA, 10 µg

Literature