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VS-FLP10020 - pwt-EosFP, FLAG®-tagged, 10µg

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pwt-EosFP is a cloning and expression vector encoding for the fluorescent green-to-red photoconvertible wildtype EosFP protein (wt-EosFP). The wildtype protein builds a tetrameric quaternary structure. It is functionally expressed in a wide range of pro- and eukaryotic cells at a temperature of 37 °C and below. wt-EosFP is particularly suitable as a localization marker of cells, tissue and subcellular compartments. The construct pwt-EosFP can be used directly in transfection and subsequent analyses by fluorescence microscopy. For labeling of subcellular compartments, short oligopeptide signals can be fused to the marker protein. The photoconversion feature makes wt-EosFP applicable to high resolution fluorescence microscopy and enables selective marking of cells and subcellular compartments.


Tracking of cells in an embryo of Xenopus laevis. Purified wt-EosFP was microinjected at stage 2. After photoconversion of a single blastomer, the fate of descendent cells can be followed by the red fluorescence.

Features

  • Expression and cloning vector for fluorescent tetrameric wt-EosFP
  • Ready-to-use construct for cell tracking
  • Green-to-red photoconvertible
  • Permanent, bright and fast
  • Applicable to high resolution fluorescence microscopy such as PALM
  • Source vector for creating fusions with signal peptides

Applications

  • Selective marking of cells and subcellular compartments by photoconversion
  • Tracking of cells and subcellular compartments

Contents

  • pwt-EosFP, FLAG-tagged, lyophilized DNA, 10 µg

Literature