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VS-FLP10030 - ptd-EosFP, FLAG®-tagged, 10µg

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ptd-EosFP is a cloning and expression vector encoding for the fluorescent green-to-red photoconvertible EosFP tandem dimer (td-EosFP), a pseudomonomeric variant in which two copies of an engineered EosFP variant are fused to form a functional monomer.
 
td-EosFP is particularly suitable as a localization marker of cells and for tracking of subcellular compartments and fusion proteins. The protein is functionally expressed in a wide range of pro- and eukaryotic cells at a temperature of 37°C and below.
 
For cell tracking, the vector construct can be used directly for transfection or microinjection and subsequent analyses by fluorescence microscopy.
 
For creating signal peptide or protein fusions, the appropriate signal sequence or the desired gene must be cloned into the provided cloning site of the ptd-EosFP vector.
 
The photoconversion feature makes ptd-EosFP applicable to high resolution fluorescence microscopy and enables selective marking of cells, subcellular compartments and fusion proteins.
 

Features

  • Expression vector for fluorescent EosFP tandemdimer (td-EosFP)
  • Ready-to-use construct for cell tracking
  • Green-to-red photoconvertible
  • Permanent, bright and fast
  • Applicable to high resolution fluorescence microscopy like PALM
  • Source vector for creating fusions with signal peptides
 

Applications

  • Tracking of subcellular compartments
  • Localization Studies of td-EosFP Fusions by Fluorescence Nanoscopy
  • Selective marking of selected mitochondria by photoconversion
 
 

Contents

  • ptd-EosFP, FLAG-tagged, lyophilized DNA, 10 µg