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Phage Display

PSKAN3 - pSKAN Phagemid Display System, anti-pIII antibody

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The monoclonal mouse anti P III (g3p) is an antibody to phage M13 coat protein pIII (g3p) monoclonal mouse IgG (Clone 10C3), Protein G purified. The antibody is supplied in PBS, pH 7.3 and supplemented with 0.2 mg/ml sodium azide. The anti-pIII (g3p) antibody is a useful analytical tool for phage display system using the coated protein III (g3-protein) for M13 for the display of recombinant proteins such as antibodies, protein – or peptide – libraries and more. See also our pSKAN Phagemid Display System.

The Epitope: the antigenic epitope of the anti-pIII (g3p) antibody resides within amino acids 292 to 302 of the mature protein III and is therefore compatible with most phagemid vectors using different N-terminal deletions of protein III.

Features

  • Antibody to M13 coat protein III (g3p)
  • Clone: 10C3
  • Monoclonal Mouse IgG

Applications


This antibody can be used in Western blots to analyze:
  • Correct reading-frame fusions of pIII-fusion proteins
  • Expression levels of pIII fusion proteins in E. coli and on the tip of the phage
  • Ratios of displayed pIII-proteins and pIII-fusion proteins on the phage-capsid
  • M13-infected E. coli strains

The epitope is also recognized under non-denaturing conditions.  Therefore, the antibody can be used for:
  • Elisa detection of PIII-fusion proteins
  • Purification of pIII-fusion proteins by affinity chromatography
  • Efficient and rapid analysis of antigen binding characteristics of recombinant antibodies selected by phage display (microplate assay)

Contents

  • anti P III (g3p) - antibody, monoclonal mouse, 100 µl

Literature