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C-MYC Tag Plasmids

OG323 - pSF-CMV-NH2-CMyc-EKT3


Starting at: $307.00

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This plasmid is compatible with all of the other DNA sequences available on this site, which are all sold in the same backbone. This flexibility allows you to create any vector you require, with a variety of functions, simply by compiling the relevant sections from our product range.

Vector: pSF-CMV-NH2-CMyc-EKT3

Product Code: OG323

Quantity Provided:  5µg

Size (bp): 4300

Bacterial Antibiotic Selection: Kanamycin

Origin and Compatibility: pUC high copy, derived from pBR322 

Copy Number: 500-700 copies per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Purpose: This vector adds a C-Myc epitope tag to the N-terminus of a protein that is encoded within the multiple cloning site. This tag allows the detection and purification of a tagged protein using antibodies raised against the C-Myc epitope. The C-Myc tag coding sequence is EQKLISEEDL. There is an enterokinase cleavage site (DDDDK) immediately downstream of the C-Myc tag that can be used to remove the C-Myc tag from a purified protein. It cleaves after the lysine residue.

Frame: The tag coding sequence is one base out of frame with the ATG start codon that is within the NcoI restriction site and was produced by deleting two base pairs adjacent to the HindIII site.

Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through our website.

Intellectual Property Status: According to our IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.

Genetic Modifications to standard parts:

  • CMV promoter – an NcoI site has been ablated in the CMV promoter. This change does not reduce expression.
  • KanR cassette – This KanR resistance region is not a wild type sequence, it has been modified to remove all restriction sites that conflict with the SnapFastTM system and to retain a high level of antibiotic resistance. It has been validated in E.coli.

Restriction site notes:

  • Bgl2, KpnI, SwaI and PmeI each cut the vector at two sites positioned to flank the promoter, start codon, origin, and KanR, respectively.
  • BsgI and BseRI recognise non-palindromic DNA sequences and cleave upstream of their recognition sites, within the stop codon that is found inside the XbaI restriction site.