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Protein A & Protein G Antibody Purification

PR-PAK002 - Protein A Starter Kit for Immunoglobulin Purification, 2 columns

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The Protein A Starter Kit uses compact affinity columns packed with immobilized Protein A to facilitate highly efficient, cost effective purification for both monoclonal and polyclonal antibodies

The immobilization of Protein A has been developed for orientation of the ligand, distribution of the ligand, stability of the immobilized ligand and elimination of non-specific surface interactions. The protein is packed into columns and immobilized on rigid, durable controlled pore glass. The internal pore size distribution results in uniform rates of diffusion and rapid transfer between solution and solid phase. The orientation and immobilization of the protein molecules on the glass surface creates high binding capacities, especially for immunoglobulins that typically do not bind well to conventional immobilized Protein A. 

Protein A has a broad species reactivity. It binds with high affinity to IgG from human (IgM, IgA, IgE, IgG1, IgG2, IgG4), cat, dog, mouse, rabbit, pig, and guinea pig.

The Protein A Starter Kit contains all components required for antibody purification including columns, buffers, ultrafiltration spin columns and tubes. Two Protein A columns are included in this kit. The columns can be stored and reused many times.

Features

  • Efficient, cost effective and rapid purification of antibodies with minimal pre-treatment
  • Kit contains all components required for antibody purification 
  • Design of the pore glass creates uniform rates of diffusion and high binding capacities
  • Protein A columns have good flow properties
  • Capacity of the Protein A columns for murine IgG at physiological pH and salt concentration is greater than other Protein A adsorbents
  • Protein A binds with high affinity to human IgG1 and IgG2 and purifies total IgG from crude protein mixtures such as serum or ascites fluid.

Applications

  • Purification of monoclonal antibodies
  • Selective purification of antibody fragments
  • Purification or removal of polyclonal IgG from serum
  • Separation of IgG sub-classes using a stepwise pH gradient 

Contents

  • Compact affinity columns with 500 µl Protein A porous glass matrix x 2
  • Binding Buffer pH 7.4 (PBS tablet for 100 ml)
  • Elution Buffer (0.1 M Glycine/HCl pH 3.0), 5ml
  • Neutralization Buffer (1 M Tris/HCl pH 8.0), 1ml
  • Luer-lock caps x 2
  • 2.5 ml reservoir x 2
  • Ultrafiltration spin filters (10 kDa) with tubes x 3
  • 2ml tubes x 10

Literature