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Protein A & Protein G Antibody Purification

PR-PAK010 - Protein A Kit for Immunoglobulin Purification, 10 columns

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The Protein A Kit (10 columns) uses compact affinity columns packed with immobilized Protein A to facilitate highly efficient, cost effective purification for both monoclonal and polyclonal antibodies

The immobilization of Protein A has been developed for orientation of the ligand, distribution of the ligand, stability of the immobilized ligand and elimination of non-specific surface interactions. The protein is packed into columns and immobilized on rigid, durable controlled pore glass. The internal pore size distribution results in uniform rates of diffusion and rapid transfer between solution and solid phase. The orientation and immobilization of the protein molecules on the glass surface creates high binding capacities, especially for immunoglobulins that typically do not bind well to conventional immobilized Protein A. 

Protein A has a broad species reactivity. It binds with high affinity to IgG from human (IgM, IgA, IgE, IgG1, IgG2, IgG4), cat, dog, mouse, rabbit, pig, and guinea pig.

This product contains 10 affinity columns with 500 µl Protein A porous glass matrix.

Features

  • Efficient, cost effective and rapid purification of antibodies with minimal pre-treatment
  • Kit contains all components required for antibody purification 
  • Design of the pore glass creates uniform rates of diffusion and high binding capacities
  • Protein A columns have good flow properties
  • Capacity of the Protein A columns for murine IgG at physiological pH and salt concentration is greater than other Protein A adsorbents
  • Protein A binds with high affinity to human IgG1 and IgG2 and purifies total IgG from crude protein mixtures

Applications

  • Purification of monoclonal antibodies
  • Selective purification of antibody fragments
  • Purification or removal of polyclonal IgG from serum
  • Separation of IgG sub-classes using a stepwise pH gradient 

Contents

  • Compact affinity columns with 500 µl Protein A porous glass matrix x 10

Literature