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Transfection for CRISPR

PNC40200 - PolyMag CRISPR Transfection Reagent, 200 µl

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PolyMag CRISPR reagent is a magnet-assisted transfection reagent optimized for delivering high levels of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For gene editing experiments, this reagent provides high transfection efficiency without toxicity. This reagent must be used with a magnetic plate.


Figure 1: PolyMag CRISPR co-transfection efficiency for CRISPR-Cas9 experiments. NIH-3t3, HeLa and MDCK cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transfection. Cells were transfected with a GFP-Cas9 plasmid and a RFP pDNA and then incubated overnight at 37oC. GFP and RFP expression was monitored 24h after transfection by fluorescence microscopy.

Features

  • High efficiency in delivery of CRISPR/Cas9 system, even on primary and hard to transfect cells
  • Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA
  • Optimized for large plasmid DNA containing Cas9 and for guide RNA
  • Low cell toxicity 

Applications

  • Genome Editing with CRISPR/Cas9

Contents

  • PolyMag CRISPR Transfection Reagent, 200 µl