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Miscellaneous DNA Vector Systems

PEG01 - Poly(His)-Tag Cloning Vector pEG-His1, 20µg

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The pEG-His1 vector was constructed for the expression of toxic gene products in E. coli. To obtain its exceptional tightness prior to induction with IPTG, the lac I repressor gene has been included in the vector and is overexpressed in plasmid-bearing cells. Recombinant fusion proteins with a 6xHis tag can be easily and selectively purified using the unique Ni-NTA (nickel-nitrilotriacetic acid) technology. The Ni-NTA affinity matrix shows an optimal binding capacity and a minimized non-specific binding resulting in highly purified and reproducible 6xHis-tagged protein preparations. 

Features

  • Optimized promoter guarantees excellent expression levels
  • Very tight expression control due to overexpression of the Lac I repressor
  • A convenient MCS allows flexible and easy cloning of the insert
  • Start codon is provided by an NdeI site
  • Inserts can be expressed as C-terminal tagged 6xHis fusion proteins for one-step purification of full length proteins by metal-chelate affinity chromatography
  • An RGS motive and the proximate Poly(His)-Tag allow detection and/or immunoprecipitation of the expressed protein with commercial anti-RGS and anti-His antibodies

Applications

  • Expression of toxic gene products in E. coli

Contents

  • pEG-His 1 vector, 5 µg
  • 5’ sequencing primer, 500 pmol
  • 3’ sequencing primer, 500 pmol

Literature