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Mitotic Checkpoint

VS-MCV00081 - pG-20-hMad1, 15μg

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This vector is a ready-to-use mammalian expression vector intended for investigation of the human mitotic checkpoint. It encodes a fusion protein of the fluorescent protein EGFP and human Mad1. These fusion proteins can be used in modern fluorescence microscopy techniques (FRET, FRAP, FCS, RICS, FCCS, and F3H) to determine localization, binding, dynamics, protein-protein interactions and protein-protein proximities. The cipher in the vector name indicates the linker length of the fusion proteins.

Features

  • Ready-to-use for live cell imaging

Applications

  • Investigation of the human mitotic checkpoint
  • Study of localization, binding, and protein-protein interactions 
  • Modern fluorescence microscopy techniques such as FRET, FRAP, FCS, RICS, FCCS, and F3H

Contents

  • pG-20-hMad1, lyophilized DNA, 15 µg

*Datasheet is available upon request*

Publications

  • Döring V. (2012). Der Zellzyklus-abhängige Aufbau des humanen Kinetochors. Dissertation FSU Jena