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Protein Gel/Blot Detection

NOWA01 - NOWA Chemiluminescent Western Detection Kit

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Starting at: $210.00

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NOWA (New Optical Western Analysis) is a detection system for HRP-conjugated antibodies in the picogram range. The sensitivity of the NOWA kit is significantly higher than that of other leading chemiluminescence kits. The NOWA kit is based on the emission of light during a reaction started by free oxygen produced by the enzymatic activity of HRP. Using a newly developed enhancer cascade, the light emission is amplified by a factor of at least one thousand. The NOWA kit contains two solutions that are combined and used for incubating the nitrocellulose or PVDF filter. After one minute of incubation time, the filter is ready to be exposed to an X-ray film. Typical exposure times range between 10 seconds and 5 minutes. The light-emitting reaction continues for about 1 hour to allow as many exposures as you may need. 

Features

  • Highly sensitive
  • Amplifies light emission by a factor of at least one thousand
  • Sufficient for 10,000 cm2 of NC or PVDF membrane
  • Stable for at least 12 months when stored at 4°C

Applications

  • Detection of HRP-conjugated antibodies in picogram range

Contents

  • NOWA kit solution, 250ml x 2

Literature