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NICE® Expression System for Lactococcus lactis

ELV00500-01 - NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in strain NZ9000, 1ml

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The Nisin controlled gene expression system (NICE®), developed at NIZO Food Research, NL, is a fully food-grade system used for controlled gene expression in Lactococcus Lactis. The NICE® system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and the tightly controlled gene expression allows the production of toxic proteins to take place. With this system, fermentation, scale-up and downstream processing are simple.

The NICE® pNZ9530 Lactococcus lactis nisRnisK vector, part of the NICE® expression system, is a low copy plasmid with pAMΒ1 origin of replication that carries the nisR and nisK genes. It is used for cloning in Lactococcus strains and in strains of other lactic acid bacteria genera that do not have the regulatory genes integrated into the chromosome. In this case, a two-plasmid system is used for nisin induced expression, such as pNZ9530 (nisRnisK) and pNZ8150 (+insert).

Please note this product requires a license for non-academic institutions. Please contact us for licensing information.

Features

  • Endotoxin-free, food-grade expression system
  • Expression of membrane proteins
  • Secretion of proteins into the medium
  • Fewer endogenous and no exogenous proteases
  • No spores or inclusion bodies
  • Tightly controlled gene expression allows production of toxic proteins
  • Simple fermentation, scale-up and downstream processing

Applications

  • Production of homologous and heterologous proteins for food, feed, pharmacological and biocatalysis applications
  • Production of prokaryotic and eukaryotic membrane proteins
  • Production of exopolysaccharides
  • Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
  • Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as, e.g., dehydrogenases and in-situ cofactor regeneration
  • High-throughput screening for enzyme evolution or enzyme comparison

Contents

  • NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000, 1ml

Literature

Publications




  • Frelet-Barrand, A., Boutigny, S., Kunji, E. R. S., und Rolland, N. (2010). "Membrane protein expression in Lactococcus lactis." Methods Mol. Biol 601, 67-85.







  • Villatoro-Hernandez, J. et al. (2012). "Heterologous protein expression by Lactococcus lactis." In A. Lorence (ed.), Recombinant Gene Expression: Reviews and Protocols, Third Edition, Methods in Molecular Biology, vol. 824. Chapter 8, p. 155 - 165.