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PMCS5 - Multiple Cloning Site Vector pMCS5

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The cloning vector pMCS5 contains the ultimate multiple cloning site (MCS) with 59 unique restriction sites. The recognition sites for many commonly used restriction enzymes are included, providing a suitable cloning site for almost any application. This vector contains 46 unique hexamer sites, 2 heptamer sites and the recognition sites for all ten known octanucleotide-specific endonucleases. The well-defined 18-mer sequence of the extremely rarely cutting enzyme I-SceI is located at one terminus of the MCS, enabling the linearization of recombinant clones. I-SceI does not recognize a palindrome and can thus be used for labeling and end-cloning. It can also be used for strand protection in unidirectional deletion experiments. Additionally, the MCS also restriction sites that occur very infrequently in human DNA such as MluI, NruI and SplI.

Features

  • MCS has 59 unique restriction sites
  • Contains 46 unique hexamer sites, 2 heptamer sites and the recognition sites for all ten known octanucleotide-specific endonucleases
  • Includes suitable cloning site for almost any application

Applications

  • Standard cloning into a site upstream of a T7 RNA polymerase promoter
  • End-cloning
  • Labeling
  • Linearization of recombinant clones
  • Blue/white selection
  • Generation of single-strand DNA (using the f1 origin)

Contents

  • pMCS5 Multiple Cloning Site Vector, lyophilized DNA, 10 µg

Literature