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Mobicols & Lab Columns

SCO500 - MobiSpin Mini Spin Column prefilled with G-50 Gel Filtration Matrix, 20/pack

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$112.00

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The MobiSpin G-Columns are prepacked with Sephadex® G-50 resin and ready-to-use. These columns remove small molecules from DNA/RNA, such as salt ions, and are therefore most suitable for buffer exchange. The Sephadex G-50 matrix is a hydrophilic matrix that swells in buffer and acquires its chromatographic properties under wet conditions. In this state, beads have a pore size of 700 Da and allow hydrated salt ions or dNTPs (dye-labeled or radiolabeled) to enter the pores while DNA/RNA under 20 bases and most other biomolecules stay outside. The MobiSpin G-Column is closed with a screw cap and the tip is a fixed snap-off plug.

Features

  • Pre-packed with Sephadex® G-50 resin
  • Equilibrated in STE buffer (10mM Tris/HCl pH 8.0, 100 mM NaCl, 1 mM EDTA)
  • Sample purification can be completed in less than 4 minutes
  • Creates reproducible results with simplified protocols
  • Numerous samples can be processed simultaneously
  • No sample dilution
  • Compatible with laboratory standards

Applications

  • Desalting of nucleic acids
  • Buffer exchange
  • Removal of dye terminators or unincorporated labeled nucleotides from DNA labeling reaction

Contents

  • MobiSpin G-50 Columns x 20

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