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DNA Extraction / DNA Purification

17289/17287/17288 - MEGAquick-spin™ Plus Total Fragment DNA Purification Kit

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MEGAquick-spin™ Plus Total Fragment DNA Purification Kit

The single kit solution for extraction of DNA fragments from agarose gels, PCR reactions and enzymatic reactions (DNA clean-up). The MEGAquick-spin™ Total Fragment DNA Purification Kit is designed to extract and purify DNA fragments of 60 bp ~ 20 kb from normal or low-melt agarose gels (in either TAE or TBE), and to purify PCR products directly from a PCR amplification and for DNA cleanup from other enzymatic reactions. Recovery of up to 95% is achieved. PCR products are commonly purified to remove excess nucleotides and primers. This multi-functional membrane-based system, which can bind up to 45µg DNA, allows recovery of isolated DNA fragments or PCR products in as little as 20 minutes, depending on the number of samples processed and the protocol used. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

NOTE: The 200 extraction size kit is available in two formats: columns with caps (for greatest protection against contamination) and columns without caps (for convenience when processing a large number of samples).

Protocol

CLICK HERE to download the User Manual