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Automated Magnetic Bead Based Purification of Nucleic Acids

MD61021 - MagSi-NGSPREP, 75ml

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Magnetic bead-based MagSi-NGSPREP offers an efficient solution for clean-up and size selection in library preparation of NGS sequencing reactions. The simple and flexible protocol can be adjusted to your specific application and NGS platform. MagSi-NGSPREP can be used manually but is also easy to automate for high-throughput processing. The separation performance is optimized with the use of validated magnetic separators for 96- or 384-well PCR plates. MagSi-NGSPREP is compatible with many different automated liquid handling systems. This product is a simple and quick way to consistent NGS results.



Gel electrophoresis of DNA ladder at different reagent:sample volume ratios. The size of fragments eluted from the beads (or that bind to the beads) is determined by the end concentration of MagSi-NGSPREP, and this in turn is determined by the mix of DNA and beads. A 50µl DNA sample plus 75µl of beads will give MagSi-NGSPREP:DNA ratio of 1.5. As this ratio is changed, the length of fragments binding and/or left in solution also changes. Hence, the lower the ratio of MagSi-NGSPREP:DNA, the larger the final fragments will be at elution. Smaller fragments are retained in the buffer, so a range of sizes can be obtained from a single sample with multiple purifications. Part of the reason for this effect is that DNA fragment size affects the total charge per molecule, with larger DNA fragments having larger charges. This promotes their electrostatic interaction with the beads and displaces smaller DNA fragments. 

Features

  • High recovery of DNA fragments of over 70%
  • Excellent removal of enzymes, primers, oligonucleotides, polymerase, and other contaminants
  • Fragment size selection adjustable between 100 and 1000 base pairs
  • Meets the requirements of common library preparation kits
  • Easy to automate

Applications

  • Clean-up in library preparation of NGS sequencing reactions
  • Size selection in library preparation of NGS sequencing reactions

Contents

  • MagSi-NGSPREP, 75ml