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Automated Magnetic Bead Based Purification of Nucleic Acids

MD60013 - MagSi-DNA cleanFIX, 400/800 purifications

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Starting at: $449.00

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Magnetic bead-based MagSi-DNA cleanFIX offers one single product for efficient PCR clean-up and dye terminator removal from DNA Sanger sequencing reactions. MagSi-DNA cleanFIX uses flexible protocols and is easy to automate for high-throughput processing. It guarantees reliable sequencing results through high average signal strength, high trace score, and long contiguous read lengths. The single magnetic bead reagent simplifies handling, system set-up and limits waste. The separation performance is optimized with the use of validated magnetic separators for 96- or 384-well PCR plates. MagSi-DNA cleanFIX is compatible with many different automated liquid handling systems.


Gel electrophoresis of PCR samples; Lanes A: unpurified PCR sample showing primer dimers; Lanes B: samples purified with MagSi-DNA cleanFIX; Lanes C: sample purified with AMPure XP; Lanes M: DNA ladder


Sequence electroferogram after purification of pGEM Plasmid Template DNA with MagSi-DNA cleanFIX


Features

  • One single product to simplify your routine
  • Allows both PCR clean-up and dye terminator removal in sequencing clean-up
  • High yield of DNA products from 80 bp up to 30 kb
  • Excellent removal of unincorporated nucleotides, primers, primer dimers, and other contaminants
  • Easy to automate

Applications

  • Dye-Terminator Removal from DNA cycle sequencing reactions 
  • PCR clean-up and purification of DNA fragments

Contents

  • MagSi-DNA cleanFIX particle mix, 4ml
  • MagSi-DNA clean Buffer P, 12ml