Home New Customer? Create Account
Member Login:

Lullaby siRNA Transfection

LL70000 - Lullaby® - siRNA Transfection Reagent

PRICE

Starting at: $214.00

Please Choose:

Size



Shipping Option



Enter Quantity:
Lullaby is the ideal siRNA transfection reagent for gene silencing. This reagent uses TEE Technology (Triggered Endosomal Escape), a mechanism that combines and exploits the properties of cationic lipids and polymers to achieve extremely efficient DNA delivery into cells. Lullaby has been successfully tested on numerous cell lines, reaching up to 90% gene silencing with high reproducibility and very low toxicity. 

RNA interference is a powerful technique to shut down genes’ expression in cells and organisms. Short RNA duplexes are extremely selective by interacting and inducing the degradation of their specific mRNA targets, and thereby inhibit the resulting protein production. Lullaby® siRNA Transfection Reagent introduces the siRNA duplexes in a variety of cells with a very high efficiency, leading to exceptional knockdown effects with low doses of siRNA.


Figure 1: GFP silencing in HeLa cells. GFP-expressing HeLa cells (A) seeded in a 24-well plate were transfected with 1μl Lullaby + 5nM (33.75ng) siRNA (B) . GFP extinction was monitored 72h post-transfection by fluorescence microscopy.


Figure 2: GFP silencing in various cell lines with Lullaby GFP-expressing cells were seeded on a 24-well plate and transfected with 10nM (67.5ng) siRNA associated with 2μl of Lullaby. GFP extinction was monitored 72h post-transfection by flow cytometry.

Features

  • Exceptional siRNA delivery efficiency and gene silencing (>90%)
  • Suitable for all siRNA applications (co-transfection, endogenous gene silencing)
  • Effective gene silencing at multiple siRNA concentrations including low doses (<10 nM), minimizing the
  • risk of off-target effects
  • Simple, rapid protocol
  • Powerful across a broad spectrum of cells and confluency (from 20 to 90%)
  • Serum compatible and non-toxic
  • Adapted to high-throughput siRNA screening  

Applications

  • siRNA transfection of cell lines
  • Gene silencing

Contents

  • LL70500 – Lullaby siRNA Transfection Reagent, 500 µl (1000 assays)
  • LL71000 – Lullaby siRNA Transfection Reagent, 1ml (2000 assays)
  • LL73000 – Lullaby siRNA Transfection Reagent, 3ml (6000 assays)