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Virus Transduction & Infection

LB00000 - LentiBlast

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Starting at: $186.00

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LentiBlast™ Kit is designed to enhance lentiviral infection and transduction in any type of cell (adherent, in suspension, primary or cell lines).

With LentiBlast, neutralizing electrostatic repulsions between membrane and viral particles and enhancing viral fusion with cell membranes can occur simultaneously. Due to a favorable “membrane permeable effect” limiting the transmembrane potential changes, LentiBlast is non-toxic and compatible with cell viability.

LentiBlast overcomes obstacles that prevent successful transduction such as cell density, passage number, lentivirus purity, and MOI. LentiBlast is also compatible with the presence of other enhancers and for sequential transductions.

Figure 1: NIH-3T3 cell line was infected with a lentivirus encoding GFP at two different MOI (0.5 and 1) in presence or not of polybrene (10 μg/mL) or two concentrations of LentiBlast (1:1000 and 1:100). Lentiviral transduction enhancer’s efficiency was determined 72 h after transduction under fluorescence microscope.

Features

  • Enhances infection and transduction efficiency of lentivirus
  • Non-toxic
  • Compatible with cell lines and primary cells
  • Requires reduced amounts of Lentivirus (low MOI)
  • Composed of two reagents for a higher compatibility and efficiency

 

Applications

  • Enhancing lentiviral transduction in cells (adherent, suspension, primary or cell lines)

 

Sizes

LB00500 (100 transductions)

  • LentiBlast™ Reagent A, 500 µl
  • LentiBlast™ Reagent B, 500 µl

LB01500 (300 transductions)

  • LentiBlast™ Reagent A, 1500 µl 
  • LentiBlast™ Reagent B, 1500 µl 

 

See Also: LentiBlast Premium Transduction Enhancer, an improved version of LentiBlast