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DNA Polymerases/SNPase

108205 - Klen SNPase, 25 U / µl 500 U

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SNPase DNA Polymerase

Klen SNPase DNA Polymerase is an optimized version of thermostable DNA polymerase designed for cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing as well as for manual DNA sequencing with 35S label. This enzyme has only 5 ‘- 3’ polymerase activity and is also recommended for SNP genotyping by allele-specific PCR (AS-PCR), allele-specific primer extension (AS-PEX) and minisequencing procedures.

The relative mutation rate during polymerization is 10-15 fold lower for Klen SNPase compared with Taq DNApolymerase. 

SNPase FEATURES

  • High specificity and very low background in allele-specific primer extension (AS-PEX), allele-specific PCR (AS-PCR) and minisequencing
  • High-fidelity incorporation of deoxy and dideoxynucleotides

SNPase APPLICATIONS

  • Automated fluorescent DNA sequencing and manual DNA sequencing 
  • Highly suited to SNP genotyping by AS-PCR, AS-PEX and minisequencing