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DNA Polymerases/SNPase, dNTPs & Kits

108205/108225 - Klen SNPase

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Starting at: $135.00

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Klen SNPase DNA Polymerase is an optimized version of thermostable DNA polymerase designed for cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing as well as for manual DNA sequencing with 35S label. This enzyme has only 5 ‘- 3’ polymerase activity and is also recommended for SNP genotyping by allele-specific PCR (AS-PCR), allele-specific primer extension (AS-PEX) and mini-sequencing procedures. The relative mutation rate during polymerization is 10-15 fold lower for Klen SNPase compared with Taq DNA polymerase. For HotStart applications, see SNPase HotStart.
 

Features

  • High specificity and very low background in AS-PEX, AS-PCR and minisequencing
  • High–fidelity incorporation of deoxy- and dideoxynucleotides
 

Applications

  • Automated fluorescent DNA sequencing 
  • Manual DNA sequencing
  • SNP genotyping by AS-PCR, AS-PEX and minisequencing
 

Sizes

108205 (500 units)

  • Klen SNPase, 25 U/µl, 500 units
  • Incomplete Reaction Buffer (5x) for PCR, 1.25ml  
  • MgCl2 100 mM, 1ml

108225 (2500 units)

  • Klen SNPase, 25 U/µl, 2500 units
  • Incomplete Reaction Buffer (5x) for PCR, 1.25ml x 2
  • MgCl2 100 mM, 1ml x 2