DNA Polymerase/Master Mixes

i-pfu DNA Polymerase is a typical heat-resistant proof-reading enzyme with 3 '→ 5' exonuclease activity. It has high fidelity and guarantees an error rate of 1x10-6 (one error rate per 13,000 base pairs). The proofreading activity of i-pfu DNA Polymerase allows PCR products with more accurate base sequences to be obtained than when using Taq DNA Polymerase. When using i-pfu DNA Polymerase, DNA fragments longer than Taq DNA Polymerase (up to 30 kb) can be amplified. Because of these properties, i-pfu DNA Polymerase is widely used in experiments in which high accuracy is required, such as gene cloning and expression. Unlike Taq DNA Polymerase, this i-pfu DNA Polymerase produces blunt-end amplification products.

Features

• High accuracy makes it possible to amplify long DNA templates 
• PCR products have more accurate bases 
• Low error rate of 1x10^-6
• Used in blunt-end Cloning
• Ideal for experiments requiring high accuracy such as gene cloning and expression

Applications

• General PCR
• Blunt Cloning
• Template amplification for DNA sequence analysis

Contents

• i-pfu DNA Polymerase (2.5 U/ml), 250 units
• 10× PCR Buffer (with 15 mM Mg²⁺), 1ml
• 10× Mg²⁺-free PCR Buffer, 1ml
• 10mM dNTPs (2.5 mM/each), 500μl 
• 25mM Mg²⁺, 1ml

Literature