i-pfu DNA Polymerase is a typical heat-resistant proof-reading enzyme with 3 '→ 5' exonuclease activity. It has high fidelity and guarantees an error rate of 1x10-6 (one error rate per 13,000 base pairs). The proofreading activity of i-pfu DNA Polymerase allows PCR products with more accurate base sequences to be obtained than when using Taq DNA Polymerase. When using i-pfu DNA Polymerase, DNA fragments longer than Taq DNA Polymerase (up to 30 kb) can be amplified. Because of these properties, i-pfu DNA Polymerase is widely used in experiments in which high accuracy is required, such as gene cloning and expression. Unlike Taq DNA Polymerase, this i-pfu DNA Polymerase produces blunt-end amplification products.
Features
- High accuracy makes it possible to amplify long DNA templates
- PCR products have more accurate bases
- Low error rate of 1x10-6
- Used in blunt-end Cloning
- Ideal for experiments requiring high accuracy such as gene cloning and expression
Applications
- General PCR
- Blunt Cloning
- Template amplification for DNA sequence analysis
Contents
- i-pfu DNA Polymerase (2.5 U/ml), 250 units
- 10Ã PCR Buffer (with 15 mM Mg2+), 1ml
- 10Ã Mg2+-free PCR Buffer, 1ml
- 10mM dNTPs (2.5 mM/each), 500μl
- 25mM Mg2+, 1ml
Literature