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DNA Polymerase/Master Mixes

25261 - i-MAX II DNA Polymerase (High Fidelity), 250u

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i-MAX ™ II DNA Polymerase is an enzyme that combines the proofreading activity of Pfu DNA Polymerase with the high processivity of Taq DNA Polymerase to improve the performance of long PCR. It can amplify long and complex DNA fragments up to 20kb from human genomic DNA and up to 30kb from a lDNA template. The accuracy of this enzyme blend reduces the number of truncated amplification products formed. i-MAX ™ II DNA Polymerase is applicable to a wide range of DNA including cloned DNA, human genomic DNA and templates that are difficult to amplify such as GC-rich or looped sequences 

Sensitivity Comparison

Figure 1: i-MAX ™ II DNA Polymerase was compared to similar products in the sensitivity of DNA amplification. Lane M, 100 bp Ladder DNA Marker; lane 1, 25 ng gDNA; lane 2, 5 ng gDNA; lane 3, 1 ng gDNA; lane 4, 200 pg gDNA; lane 5, 40 pg gDNA; lane 6, 8 pg gDNA; lane 7, 1.6 pg gDNA; lane NC, negative control

Batch Stability

Figure 2: Production of i-MAX ™ II DNA Polymerase Batch variation was confirmed through sensitivity activity of 1 Kb size using Lamda DNA as a template. Lane M, 1 Kb DNA ladder; lane NC, Negative control; lane 1, 100 ng lamda DNA; lane 2, 50 ng lamda DNA; lane 3, 25 ng lamda DNA; lane 4, 12.5 ng lamda DNA; lane 5, 6.25 ng lamda DNA; lane 6, 3.125 ng lamda DNA; lane 7, 1.5625 ng lamda DNA

Features

  • Utilizes the advantages of both Pfu DNA Polymerase and Taq DNA Polymerase
  • Increases the fidelity and yield of PCR amplification
  • Improves the performance of long PCR
  • High accuracy reduces the number of truncated amplification products 

Applications

  • Standard and long PCR
  • PCR with difficult templates
  • TA Cloning
  • Blunt Cloning

Contents

  • i-MAX™ II DNA Polymerase (5 U/ml), 250 units
  • 10× PCR Buffer (with 20mM Mg2+), 1.5ml
  • 10× Mg2+-free PCR Buffer, 1.5ml
  • 10mM dNTPs (2.5mM/each), 800μl 
  • 25mM Mg2+, 1.5ml  

Literature