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Endoproteinases

EP0205 - IgA Protease (Igase Pro-Pro-Y-Pro), 50μg

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Phenylboronate columns



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IgA Protease (Igase Pro-Pro-Y-Pro) is used for the sequence-specific enzymatic cleavage of fusion proteins in recombinant protein technology. Endoprotease Pro-Pro-Y-Pro, a protein of 106 kd, efficiently processes polypeptides at authentic or engineered sites. Since the endoprotease cleaves the proline-rich hinge region of IgA1, the enzyme is also referred to as Igase or IgA protease. The highly specific proteolysis can be obtained not only with soluble and purified protein fusions, but also with insoluble aggregates derived from cytoplasmic inclusion bodies. Additionally, the affinity of endoproteinase Pro-Pro-Y-Pro to Phenylboronate columns facilitates separation of enzyme and substrate after digestion.

Features

  • Highly efficient specific endoprotease 
  • Cleaves amino acid sequence N-X-Z-Pro-Pro/-Y-Pro-C (X = preferred Pro or Ser; Y = Thr, Ser or Ala; Z = preferred Arg or Thr) 
  • Cleaves insoluble aggregates derived from inclusion bodies
  • Natural substrate is IgA1 

Applications

  • Cleavage of fusion proteins

Contents

  • IgA Protease (Igase Pro-Pro-Y-Pro), recombinant, 50 µg

Literature