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BSUB PGRAC01 VECTORS - Bacillus subtilis Pgrac01 vectors, 10µg

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B. subtilis vector



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The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins. The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.
 
Multiple B. subtilis vectors are offered:
 
  • pHT01 (PBS001) – allows high-level expression of recombinant proteins within the cytoplasm. Like pHT43, this vector is based on the strong σA -dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTGinducible) promoter by addition of the lac operator. 
 
  • pHT43 (PBS002) – allows high-level expression of recombinant proteins within the cytoplasm; directs the recombinant proteins into the medium. Like pHT01, this vector is based on the strong σA -dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTGinducible) promoter by addition of the lac operator. 
 
  • pHT08 (PBS003) – derivative of pHT01 with an 8xHis tag
 
  • pHT09 (PBS004) - derivative of pHT01 with a Strep tag
 
  • pHT10 (PBS005) - derivative of pHT01 with a c-Myc tag
 
 

Vector

Cat. #

Tag

Secretion

Sequence

Vector map

pHT01

PBS001

 

 

Link

Link

pHT43

PBS002

 

Yes

Link

Link

pHT08

PBS003

8xHis Tag

 

Link

Link

pHT09

PBS004

Strep Tag

 

Link

Link

pHT10

PBS005

c-Myc Tag

 

Link

Link

 
 
Please note these products require a license for non-academic institutions. Please contact us for licensing information.
 

Features

  • Non-pathogenic
  • No significant bias in codon usage
  • Can secrete functional extracellular proteins directly into the culture medium
  • Useful for the construction of metagenomic libraries
 

Applications

  • Host for gram-positive bacteria
 

Vectors

  • PBS001 - pHT01 vector, lyophilized plasmid DNA, 10 µg
  • PBS002 - pHT43 vector, lyophilized plasmid DNA, 10 µg
  • PBS003 - pHT08 vector, lyophilized plasmid DNA, 10 µg
  • PBS004 - pHT09 vector, lyophilized plasmid DNA, 10 µg
  • PBS005 - pHT10 vector, lyophilized plasmid DNA, 10 µg
 

Literature

 

Publications

                   

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