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Master Mixes

119550 - HotRox Master Mix, high Rox; 200 reactions

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HotRox Master Mixes are optimized ready-to-use mixtures for the amplification and detection of DNA in Real Time quantitative PCR (qPCR). ROX reference dye is included in the HotRox Master Mixes to normalize the fluorescent signal on compatible instruments. HotRox Master Mixes contain Bioron's SuperHotTaq DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HotRox Master mix can be used in all Real-Time devices that contain a filter for ROX dye. The concentration of ROX depends on the instrument used.

Features

  • High sensitivity and specificity
  • Bioron’s SuperHotTaq DNA Polymerase included
  • ROX reference dye included to normalize the fluorescent signal on compatible instruments

Applications

  • Real Time PCR
  • qPCR
  • End point analysis

Contents

  • HotRox Master Mix (2x)*, 1.25ml x 2 
  • MgCl2 100 mM, 1ml
  • PCR water, 1.8ml x 2
*Contains Antibody blocked Hotstart Taq DNA Polymerase (recombinant), PCR Buffer with 3 mM MgCl2, 400 µM each dNTP and 0.1 µM ROX reference dye. 


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