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DNA Extraction / DNA Purification

17046 - G-spin™ Total DNA Extraction Kit

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G-spin™ Total DNA Extraction Kit 

The single kit solution for all your genomic DNA requirements. The G-spin™ Total DNA Extraction Kit provides efficient methods for purification of total DNA from cultured animal cells, animal tissue, rodent tails, fixed tissue, animal hair, gram negative bacteria and blood samples for reliable PCR and Southern blotting. Purified DNA can be up to 50kb in size, with fragments of 20-30kb predominating.

Protocol

protocol

Description

  • G-spin Total DNA Extraction Kit yields pure DNA ready for direct amplification in 20-30 minutes.
    • Purification does not require phenol/chloroform extraction or alcohol precipitation
    • DNA is eluted in buffer or water, ready for direct addition to PCR or other enzymatic reactions
    • DNA can also be stored at -20oC for later use
    • DNA is free of protein, nucleases and inhibitors
    • Fragments range from 20kb up to 50kb
  • Specific protocols provided for:
    • Type A Protocol: blood, body fluids
    • Type B Protocol: tissues, rodent tail
    • Type C Protocol: cell, buffy coat
    • Type D Protocol: dried blood spots
    • Type E Protocol: fixed tissues
    • Type F Protocol: bacteria
    • Type G Protocol: biological swabs
    • Type H Protocol: animal hair

CLICK HERE to download the User Manual


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