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GRiSP Research Solutions - PCR & qPCR, RNA Research, and Sample Purification

GK26.0050 - GRS FullSample Purification Kit, 50 preps/kit

PRICE

$474.00

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The GRS FullSample Purification Kit provides an efficient and fast method for the simultaneous purification of genomic DNA, total RNA (including miRNA), and total protein from whole blood and other biological fluids, animal tissues, and cultured cells. This kit is ideal for limited samples.

With this kit, the entire procedure of DNA and RNA purification can be completed in less than 30 minutes without phenol/chloroform or alcohol precipitation. DNA is bound to a DNA binding spin column and the flow-through is subsequently passed through a RNA binding spin column. The proteins in the flow-through can then be precipitated with acetone. Contaminants are washed, and DNA is eluted with a low salt buffer. RNA is eluted with RNAse-free water. 

Eluted gDNA (20-30kb) is suitable for most common downstream applications, including PCR, whereas purified total RNA (including microRNA) is ready to use for RT-PCR, primer extension, and Northern Blotting. After protein precipitation, the pellet is washed with ice-cold ethanol and re-suspended in an appropriate volume of buffer compatible with the downstream application of choice.

Features

  • Sample Size = up to 200 µl of biological fluids, 500 µl of whole blood, 25 mg of animal tissue, or 5x106
  • cultured animal cells
  • Expected Yield = typically up to 9 µg of pure genomic DNA, 20 µg of total RNA, and 120 µg of protein
  • (from 1.5x106 HeLa cells)
  • Elution Volume = 30-200 μl for DNA, 25-50 µl for RNA, protein resuspension in desired volume
  • Operation Time = 25 minutes for DNA & RNA, approximately 1 hour for protein precipitation

Applications

  • Purification of genomic DNA, total RNA (including miRNA), and total protein from whole blood and other biological fluids, animal tissues, and cultured cells

Contents

  • Red blood cell lysis buffer, 100ml
  • DRP lysis buffer, 30ml
  • DNA wash buffer 1, 45ml
  • DNA wash buffer 2, 12.5ml
  • Wash buffer RNA 1, 30ml
  • Wash buffer RNA 2, 25ml
  • RNase-free water, 6ml x 2
  • 8M Urea, 6ml
  • Genomic DNA mini spin column x 50
  • RNA mini spin column x 50
  • 2ml collection tube x 200
  • 1.5ml microtube (DNase/RNase-free) x 150
  • DNase I solution, 275 µl
  • DNase I reaction buffer, 2.5ml