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Master Mixes

119200 - GreenHot Master Mix

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GreenHot Master Mix

GreenHot Master Mix (qPCR 2x) is optimized for Sybr Green I Assays and contains all the components necessary to perform Real Time qPCR. Only template and primers should be added. The GreenHot Master Mix (qPCR 2x) contains optimal levels of active Sybr Green I dye and SuperHotTaq DNA polymerase that enables detection of low copy number targets.

Features:
  • High sensitivity & specificity
  • Optimized buffer containing active Sybr Green I dye
  • Reproducibility 
  • Broad dynamic range
Applications:
  • Real Time PCR
  • qPCR
  • End point analysis
Performance and purity tests:
  • Tested for the absence of endodeoxyribonucleases and exodeoxyribonucleases.
  • The GreenHot Master Mix is tested in the amplification of a single-copy gene of mouse genomic DNA
  • Endodeoxyribonuclease Assay: No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 25µl of GreenHot Master Mix with 1µg of pUC19 DNA in 50µl for 4 hours neither at 37C nor at 70C.