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Genomic DNA Extraction

17231 - G-DEX IIc Genomic DNA Extraction Kit for Cells and Tissues, 300 preps

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The G-DEX™IIc Genomic DNA Extraction Kit (for Cell/Tissue) is a rapid and efficient method for isolation of high molecular weight genomic DNA from gram-negative bacteria, animal cells and tissues of all types. The protocol for most samples includes only 5 steps and can be completed in less than 1 hour. Sample sizes can range from a single cell to 1 gram of tissue. This kit is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications.

Features

  • Can be used with a wide range of biological samples
  • Isolates genomic DNA in only 20-60 min 
  • Easy-to-use; steps include lysis → protein removal → DNA precipitation → DNA hydration 
  • Recovers up to 20 μg from cells (1 ~ 206 cells) or 35 μg from tissues (~20 mg tissue) 
  • High purity: Extracted DNA ratio (OD260/280) = 1.9 ~ 2.1 (free from contaminants)

Applications

  • PCR & Real-time PCR
  • Southern blotting
  • SNP
  • Gene cloning
  • Genotyping
  • Pathogen research 

Contents

  • Lysis buffer, 60ml
  • PPT buffer, 25ml
  • DNA rehydration buffer, 25ml
  • RNase A (lyophilized), 3mg

Literature