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Protein Markers and Ladders

PS1000 - FluoroStain Protein Fluorescent Staining Dye (Red 1000X), 1ml

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The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain™ Protein Fluorescent Staining Dye binds to proteins with high specificity, making de-staining process an option rather than a requirement. With further reduction of background signals via de-staining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. 

In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience. The stained gel can be visualized with blue-light illumination, avoiding the risk of skin/eye damage caused by UV light. For best results, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×).

Spectral Characteristics

When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm. In the absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.

These spectral characteristics make this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.

Compatibility to MASS analysis: Comparison of MALDI-TOF mass spectra of bovine serum albumin (BSA) stained with FluoroStain Protein Fluorescent Staining Dye (A) and with Coomassie Blue (B). BSA proteins are seperated on SDS-PAGE, stained with fluorescent dye or conventional Coomassie Blue, followed by trypsin digestion in gel, and then analyzed by MALDI-TOF. 

Features

  • Alternative to Coomassie Blue protein staining method with greater sensitivity and ease of operation
  • Does not require de-staining process
  • High sensitivity and specificity
  • Safer for user as the stained gel can be visualized with blue-light illumination rather than UV light
  • Compatible with a wide variety of gel reading facilities
  • Excitation peaks at 369 and 517 nm and emission at 605 nm

Contents

  • PS1000 FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×), 1ml