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DNA Loading Dyes and Stains

DL3000 - ExcelDye™ 6x DNA Loading Dye, Blue, 5ml x 2

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ExcelDye™ 6× DNA Loading Dye (Blue) is a pre-mixed buffer for tracking DNA samples during electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Features

  • Composed of 0.03% Xylene cyanol FF, 0.03% Bromophenol blue, 10 mM Tris-HCl (pH 8.0), 60% glycerol, 60 mM EDTA

Applications

  • Tracking DNA migration during electrophoresis

Contents

  • DL3000 ExcelDye 6× DNA Loading Dye (Blue), 5ml x 2