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Protein Markers and Ladders

PM2500 - ExcelBand 3-color Regular Range Protein Marker, 250ul x 2

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The PM2500 ExcelBand™ 3-color Regular Range Protein Marker is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). 

Features

  • Three-color protein standard with 10 pre-stained proteins
  • Ready-to-use
  • Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol)

Applications

  • Monitoring protein separation during SDS-polyacrylamide gel electrophoresis
  • Verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) 
  • Approximating size of proteins

Contents

  • PM2500 ExcelBand™ 3-color Regular Range Protein Marker, 250 µl x 2