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Protein Markers and Ladders

PM5200 - ExcelBand 3-color Pre-Stained Protein Ladder Broad Range, 250ul x 2

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The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). 

Features

  • Three-color protein standard with 15 pre-stained proteins
  • Ready-to-use
  • Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol)

Applications

  • Monitoring protein separation during SDS-polyacrylamide gel electrophoresis
  • Verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) 
  • Approximating size of proteins

Contents

  • PM5200 3-color Pre-Stained Protein Ladder Broad Range, 250 µl x 2