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Protein Markers and Ladders

PM2610 - Enhanced 3-color High Range Protein Marker (9-245 kDa)

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The PM2610 ExcelBand™ Enhanced 3-color High Range Protein Marker is a ready to use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine buffer (9 to 235 kDa in BisTris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

Features

  • Three-color protein standard with 12 pre-stained proteins
  • Ready-to-use, supplied in gel buffer
  • Approximately 0.2~0.6 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 3.6 M Urea, and 15 % (v/v) Glycerol)

Applications

  • Monitoring protein separation during SDS-polyacrylamide gel electrophoresis
  • Verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) 
  • Approximating size of proteins

Contents

  • PM2610 ExcelBand™ Enhanced 3-color High Range Protein Marker, 250 µl × 2

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