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Mycoplasma Detection

25235 - e-Myco Mycoplasma PCR Detection Kit, 48 tests


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e-Myco Mycoplasma PCR Detection Kit

48 x 20μl reactions

Mycoplasma is a common and serious contaminant of cell cultures. It has been shown that 30-87% of cell cultures are infected with Mycoplasma. In continuous cell cultures, contaminating Mycoplasma may grow slowly, not killing the cells outright but affecting various parameters, leading to unreliable results. Testing for Mycoplasma is an important and necessary quality control measure.

The primers used in this Mycoplasma detection kit anneal to conserved regions of the Mycoplasma genome, allowing detection of all important species of Mycoplasma (including M. opalescens, M. arginini; M. fermentans; M. caviae, M. hyorhinis, M. indiense, M. orale, Acholeplasma laidlawii and many more - see User Manual). Mycoplasma contaminants can be detected simply as amplified fragments on a DNA gel; labeled probes are not necessary.

Each of the 48 e-Myco Mycoplasma PCR detection tubes (6 x 8-strips) contains all the required components (i-StarTaq DNA Polymerase, dNTPs, 1 x buffer and Mycoplasma primers) - you simply add your sample (DNA or cells) to each tube and run the reaction.

NEW: each 8-strip is individually vaccum-packed for long shelf life.


e-Myco Mycoplasma PCR Detection Kit Features:

  • High Sensitivity: The kit allows detection of 10 infected cells or 10ng genomic DNA
  • Simplicity: Detects all important strains of Mycoplasma with a single protocol
  • Broad Detection Range: Use of primers which anneal to conserved regions of the genome allow detection of all important Mycoplasma specie
  • Species Determination: You can determine the species of mycoplasma by sequencing the amplified products

Now Ver. 2.0 includes:

  • an internal control to reduce the chance of false negatives
  • the ECC system (Elimination of Carryover Contamination)