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DreamFect featuring Tee-Technology

DF-40000 - Dreamfect Transfection Reagent with Tee Technology

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Starting at: $195.00

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DreamFect™ Transfection Reagent uses TEE (Triggered Endosomal Escape) technology to achieve efficient nucleic acid delivery into cells. It contains both a lipidic portion and a cationic portion, allowing the association of nucleic acids with the reagent and permitting nucleic acid delivery into cells. Due to its unique properties, DreamFect™ releases large amounts of DNA into the cytosol, increasing DNA nuclear delivery. Consequently, DreamFect™ is highly efficient at low doses of nucleic acids. Fewer cationic lipids are used and the potential secondary effects are avoided.


Figure 1: Protocol. Tee-Technology combines and exploits the properties of cationic lipids and polymers to achieve an extremely efficient DNA delivery into cells. 

Figure 2: DreamFect transfection efficiency in various cell lines. 1x105 cells were transfected with 0.5 μg / well of pEGFP plasmid DNA in 24-well plates. Percentage of transfected cells was measured 24h post transfection by flow cytometry.


Figure 3: Comparison of DreamFect efficiency with other transfection reagents. Cell lines (1x105 cells / well) were transfected with 1 μg of pLuc plasmid DNA and 3 μl of DreamFect Gold reagent per well in a 24-well plate. The other transfection reagents were assayed according to the manufacturer’s instructions.

Features

  • High efficiency and reproducibility
  • Can be used with all nucleic acids and all cells including suspension cell lines
  • Ideal for all nucleic acids applications
  • Serum-compatible
  • Non-toxic and biodegradable
  • Simple, rapid protocol

Applications

  • Transfection of cell lines 

Sizes

  • DF40500 – DreamFect Transfection Reagent, 500 µl (125-250 transfections)
  • DF41000 – DreamFect Transfection Reagent, 1ml (250-500 transfections)
  • DF45000 – DreamFect Transfection Reagent, 5ml (1250-2500 transfections)

See also: DreamFect Stem