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DreamFect Stem Cell Transfection

ST30500 - DreamFect Stem Cell Transfection Reagent

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DreamFect™ Stem is an original, simple and efficient reagent specially developed to transfect multipotent and embryonic stem cells with high transfection efficiency and superior transgene expression level. DreamFect™ Stem transfection reagent is based on TEE Technology (Triggered Endosomal Escape) that combines and exploits the properties of cationic lipids and polymers to achieve an extremely efficient DNA delivery into stem cells. It is a powerful reagent with improved cytoplasmic release and better biodegradability. DreamFect™ Stem allows DNA delivery in a variety of stem cells.

DreamFect™ Stem exhibits high transfection efficiency on human adult multipotent stem cells such as MSC (Mesenchymal Stem Cells), AdSC (Adipose derived Stem Cells), AFSC (Amniotic Fluid Stem Cells), and KG1a (leukemic stem-like cells) as well as embryonic stem cells.


Figure 1: Several human stem cells (MSC, AdSC, AFSC, KG1a) were transfected with 0.5 μg of pVectOZ-GFP plasmid DNA and 1.5 μl per well of DreamFect™ Stem reagent per well in a 24-well plate. Percentage of GFP positive cells were measured 48h post transfection by flow cytometry.



Figure 2: Adherent stem cells (MSC, AdSC, AFSC) were transfected with 0.5 μg of pVectOZ-GFP plasmid DNA and 1,5 μl of Dreamfect™ Stem per well in a 24-well plate. Transfection efficiency was assessed by fluorescence microscopy 48h post transfection (Magnification x 200). 

Features

  • High transfection efficiency for multipotent Stem cells
  • Minimized toxicity due to reagent biodegradability and low DNA amount required
  • Cell phenotype and differentiation potential are not affected
  • Serum Compatible
  • Simple, rapid protocol

Applications

  • Transfection of stem cells with high transgene expression level

Sizes

  • ST30500 – DreamFect™ Stem, 500 µl (125-250 transfections)
  • ST31000 – DreamFect™ Stem, 1ml (250-500 transfections)