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DNA Polymerases/SNPase

101005 - DFS Taq DNA Polymerase DNA FREE! 500 units

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DFS-Taq DNA Polymerase is a unique thermostable DNA Polymerase of approximately 94 kDa isolated from Thermus aquaticus strain YT-1. This unmodified Taq DNA Polymerase replicates DNA at 72°C. The DFS-Taq Polymerase catalyzes the polymerization of nucleotides into duplex DNA in 5’ – 3’ direction in the presence of magnesium ions and possesses a 5’ – 3’ exonuclease activity. The DNA-free Taq Polymerase is highly purified and is free of nonspecific endo- or exonucleases. 

Features

  • Free from bacterial DNA
  • Universal buffer system for different applications
  • High sensitivity: > 6 molecules template
  • Efficient amplification of single copy genes
  • High product yield

Applications

  • Amplification of DNA fragments up to 5 kb
  • Amplification of single-copy genes
  • Labeling of PCR products with modified nucleotides (biotin-dUTP, fluorescein-dUTP)
  • Cycle sequencing
  • Generation of PCR products for TA-cloning
  • Detection of bacterial pathogens

Contents

  • DFS-Taq DNA Polymerase, 500 units
  • Incomplete NH4 Reaction Buffer (10x), 1.8ml
  • Complete NH4 Reaction Buffer (10x), 1.8ml
  • Complete KCl Reaction Buffer (10x), 1.8ml
  • MgCl2 100 mM, 1ml

Other Sizes Available: 2500 units10,000 units

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