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PBBR02 - Broad-Host-Range Vector pBHR1 (mobilizable)

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pBHR1 is a mobilizable vector that was created by removing a frame shift in the mobilization gene of pBBR122, thereby restoring the gene function. All other features of pBHR1 are similar to PBBR122. 

pBBR122 is a cloning vector with very broad host-range maintenance. As opposed to other known broad-host-range vectors, it replicates at medium copy numbers and has a small size (5305 bp). This greatly facilitates genetic studies of a wide variety of Gram-negative bacteria and makes pBBR122 particularly interesting for studies of broad-host-range replicons. pBBR122 has shown stable replication in all Gram-negative organisms that have been tested. pBBR122 was derived from pBBR1, which was isolated from Bordetella bronchiseptica S87. It is compatible with other broad-host-range vectors, as it does not belong to any of the broad-host-range incompatibility groups IncP, IncQ or IncW.

Features

  • Replicates at medium copy numbers and has a small size
  • Broad host-range maintenance

Applications

  • Cloning and gene expression in bacteria other than E. coli (mainly Gram-negative)
  • Studies of broad-host-range replicons
  • Studies of Gram-negative bacteria
  • Changing bacteria hosts

Contents

  • pBHR1 vector DNA (mobilizable), lyophilized, 5 µg

Literature